Journal: Cell reports

Article Title: ZBTB7A is a modulator of KDM5-driven transcriptional networks in basal breast cancer

doi: 10.1016/j.celrep.2024.114991

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-MTA2 , Cell Signaling Technology , Cat# 15793.

Techniques: Control, Produced, Recombinant, Virus, XF Assay, ROS Assay, CRISPR, Mass Spectrometry, Knock-Out, Plasmid Preparation, Software, Combined Bisulfite Restriction Analysis Assay, Genome Wide

Journal: Cell reports

Article Title: ZBTB7A is a modulator of KDM5-driven transcriptional networks in basal breast cancer

doi: 10.1016/j.celrep.2024.114991

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Antibodies used for Immunoblotting were anti-beta-Actin (Sigma, A2228), anti-alpha Tubulin (Sigma, T5168), anti-ZBTB7A (Invitrogen, 14-3309-82), anti-KDM5A (Abcam, ab70892), anti-KDM5B (Sigma, HPA027179), anti-KDM5C (Abcam, ab34718), anti-H3K4me3 (Abcam, ab8580), anti-H3 (Active Motif, 39763), anti-RHOA (Cell Signaling Technology, 2117), anti-PKN2 (Abcam, ab87812), anti-MTA1 (Cell Signaling Technology, 5647), anti-MTA2 (Cell Signaling Technology, 15793), anti-MBD2 (Abcam, ab188474), anti-MBD3 (Cell Signaling Technology, 14540), anti-CHD3 (Cell Signaling Technology, 4241), anti-CHD4 (Cell Signaling Technology, 11912), anti-phospho-p65 (Ser536) (Cell Signaling Technology, 3033), anti-p65 (Abcam, ab32536), anti-phospho-p50 (Ser337) (Invitrogen, PA5–37658), anti-p50 (Invitrogen, MA5–15870), Goat anti-Mouse IgG Secondary HRP (Invitrogen, 62–6520), Goat anti-Rabbit IgG Secondary HRP (Invitrogen, 65–6120), and Goat anti-Armenian Hamster IgG Secondary HRP (Invitrogen, PA1–32045).

Techniques: Control, Produced, Recombinant, Virus, XF Assay, ROS Assay, CRISPR, Mass Spectrometry, Knock-Out, Plasmid Preparation, Software, Combined Bisulfite Restriction Analysis Assay, Genome Wide

Journal: Heliyon

Article Title: Mechanism of HIF-1α promoting proliferation, invasion and metastasis of nasopharyngeal carcinoma by regulating MMP-2 in hypoxic microenvironment

doi: 10.1016/j.heliyon.2024.e40760

Figure Lengend Snippet: Primer sequences used in this study.

Article Snippet: 10 % skimmed milk powder (solarbio, China) was sealed at room temperature for 2 h. The primary antibodies HIF-1α (1:1000, Abcam, USA), MMP-2 (1:1000, Cohesion Biosciences, UK), Fas (1:1000, Abcam, USA), BRCA1 (1:1000, Cohesion Biosciences, UK), TIMP-1 (1:1000, Abcam, USA), AKT1 (1:1000, Cohesion Biosciences, UK), VEGFA (1:1000, Abcam, USA), MET (1:1000, Cohesion Biosciences, UK), MMP-9 (1: 1000, Cohesion Biosciences, UK), MTA2 (1:1000, Cohesion Biosciences, UK), and β-actin (1:5000, Cohesion Biosciences, UK) were incubated overnight at 4 °C.

Techniques: Sequencing

Journal: Heliyon

Article Title: Mechanism of HIF-1α promoting proliferation, invasion and metastasis of nasopharyngeal carcinoma by regulating MMP-2 in hypoxic microenvironment

doi: 10.1016/j.heliyon.2024.e40760

Figure Lengend Snippet: Genes up-regulated or down-regulated at least 2.0-fold by silencing HIF-1α in HONE1 cells.

Article Snippet: 10 % skimmed milk powder (solarbio, China) was sealed at room temperature for 2 h. The primary antibodies HIF-1α (1:1000, Abcam, USA), MMP-2 (1:1000, Cohesion Biosciences, UK), Fas (1:1000, Abcam, USA), BRCA1 (1:1000, Cohesion Biosciences, UK), TIMP-1 (1:1000, Abcam, USA), AKT1 (1:1000, Cohesion Biosciences, UK), VEGFA (1:1000, Abcam, USA), MET (1:1000, Cohesion Biosciences, UK), MMP-9 (1: 1000, Cohesion Biosciences, UK), MTA2 (1:1000, Cohesion Biosciences, UK), and β-actin (1:5000, Cohesion Biosciences, UK) were incubated overnight at 4 °C.

Techniques: Control, Transduction

Journal: Heliyon

Article Title: Mechanism of HIF-1α promoting proliferation, invasion and metastasis of nasopharyngeal carcinoma by regulating MMP-2 in hypoxic microenvironment

doi: 10.1016/j.heliyon.2024.e40760

Figure Lengend Snippet: Confirmation of genes regulated by HIF-1α in HONE1. A. qRT-PCR confirmation of genes regulated by HIF-1α in HONE1, including FAS, BRCA1, TIMP-1, AKT1, VEGFA, MET, MMP-2, MMP-9, MTA2. B. Western blot confirmation of genes regulated by HIF-1α in HONE1, including Fas, BRCA1, TIMP-1, AKT1, VEGFA, MET, MMP-2, MMP-9, MTA2. Full, non-adjusted images of the WB are shown in .

Article Snippet: 10 % skimmed milk powder (solarbio, China) was sealed at room temperature for 2 h. The primary antibodies HIF-1α (1:1000, Abcam, USA), MMP-2 (1:1000, Cohesion Biosciences, UK), Fas (1:1000, Abcam, USA), BRCA1 (1:1000, Cohesion Biosciences, UK), TIMP-1 (1:1000, Abcam, USA), AKT1 (1:1000, Cohesion Biosciences, UK), VEGFA (1:1000, Abcam, USA), MET (1:1000, Cohesion Biosciences, UK), MMP-9 (1: 1000, Cohesion Biosciences, UK), MTA2 (1:1000, Cohesion Biosciences, UK), and β-actin (1:5000, Cohesion Biosciences, UK) were incubated overnight at 4 °C.

Techniques: Quantitative RT-PCR, Western Blot